Citation:
Abstract:
The ability to chronically record from populations of neurons in freely behaving animals has proven an invaluable tool for dissecting the function of neural circuits underlying a variety of natural behaviors, including navigation(1) , decision making (2,3), and the generation of complex motor sequences(4,5,6). Advances in precision machining has allowed for the fabrication of light-weight devices suitable for chronic recordings in small animals, such as mice and songbirds. The ability to adjust the electrode position with small remotely controlled motors has further increased the recording yield in various behavioral contexts by reducing animal handling.(6,7) Here we describe a protocol to build an ultra-light motorized microdrive for long-term chronic recordings in small animals. Our design evolved from an earlier published version(7), and has been adapted for ease-of use and cost-effectiveness to be more practical and accessible to a wide array of researchers. This proven design (8,9,10,11) allows for fine, remote positioning of electrodes over a range of ~ 5 mm and weighs less than 750 mg when fully assembled. We present the complete protocol for how to build and assemble these drives, including 3D CAD drawings for all custom microdrive components.
November 2012 (pdf).
Notes:
Otchy, Timothy MOlveczky, Bence Peng1R01NS066408-01A1/NS/NINDS NIH HHS/T32 MH020017/MH/NIMH NIH HHS/Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tVideo-Audio Media2012/11/22 06:00J Vis Exp. 2012 Nov 8;(69). pii: 4314. doi: 10.3791/4314.